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Macromolecule Polymer Size Detection
The method of measuring the size of polymeric polymers
If you want to accurately measure the size of polymeric polymers, you must use scientific methods and be careful.
First, be reasonable. Polymer polymers have complex structures with different sizes, either linear, branched, or reticulated. To know their exact size, you need to know the stretching, curling, and aggregation states of their molecular chains. The principle of this is related to intermolecular forces, solvent effects, and many other factors.
Second, choose the appropriate method. One is gel permeation chromatography (GPC). This is a commonly used technique that uses a porous gel as a stationary phase, and the sample passes through the chromatographic column with the mobile phase. Depending on the size of the molecule, the macromolecule comes out of the column first, and the small molecule comes out of the column later. With the correction of the standard sample, the molecular weight and molecular weight distribution of the polymer can be obtained, which is the key to measuring its size. The second is the light scattering method. When light is irradiated on the polymer solution, the light is scattered due to the different refractive indices of the molecule and the solvent. By measuring the parameters such as the intensity and angle of the scattered light, the size and shape of the molecule can be inferred. And the dynamic light scattering method can better detect the Brownian motion of the molecule, obtain the diffusion coefficient, and then calculate the particle size.
Furthermore, it is necessary to be careful. In the operation of GPC, the selection of the chromatographic column, the configuration of the mobile phase, and the preparation of the sample all need to be accurate. The column temperature and flow rate must also be constant to avoid affecting In the light scattering method, the purity of the solution and the stability of the testing environment are all related to the accuracy of the results.
In short, to obtain accurate data on the size of polymers, it is necessary to understand, select, and operate carefully. The three complement each other to achieve the essence of testing.
If you want to accurately measure the size of polymeric polymers, you must use scientific methods and be careful.
First, be reasonable. Polymer polymers have complex structures with different sizes, either linear, branched, or reticulated. To know their exact size, you need to know the stretching, curling, and aggregation states of their molecular chains. The principle of this is related to intermolecular forces, solvent effects, and many other factors.
Second, choose the appropriate method. One is gel permeation chromatography (GPC). This is a commonly used technique that uses a porous gel as a stationary phase, and the sample passes through the chromatographic column with the mobile phase. Depending on the size of the molecule, the macromolecule comes out of the column first, and the small molecule comes out of the column later. With the correction of the standard sample, the molecular weight and molecular weight distribution of the polymer can be obtained, which is the key to measuring its size. The second is the light scattering method. When light is irradiated on the polymer solution, the light is scattered due to the different refractive indices of the molecule and the solvent. By measuring the parameters such as the intensity and angle of the scattered light, the size and shape of the molecule can be inferred. And the dynamic light scattering method can better detect the Brownian motion of the molecule, obtain the diffusion coefficient, and then calculate the particle size.
Furthermore, it is necessary to be careful. In the operation of GPC, the selection of the chromatographic column, the configuration of the mobile phase, and the preparation of the sample all need to be accurate. The column temperature and flow rate must also be constant to avoid affecting In the light scattering method, the purity of the solution and the stability of the testing environment are all related to the accuracy of the results.
In short, to obtain accurate data on the size of polymers, it is necessary to understand, select, and operate carefully. The three complement each other to achieve the essence of testing.
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